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Registros recuperados: 102
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Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas ArchiMer
Asmani, Katia; Petton, Bruno; Le Grand, Jacqueline; Mounier, Jerome; Robert, Rene; Nicolas, Jean-louis.
This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5‰ in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 105 ml− 1) and in larvae (103 larva− 1) on day 15. Bacterial...
Tipo: Text Palavras-chave: RAS; Bivalve larvae; Microbiota; Pyrosequencing; Hatchery.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00346/45691/45311.pdf
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Estimation de la teneur en acétate de tributyle étain dans l'eau de mer, par une méthode biologique ArchiMer
His, Edouard; Maurer, Daniele; Robert, Rene.
L’acétate de tributyle-étain, composé actif de certaines peintures antisalissures, représente un danger certain pour les zones conchylicoles de captage (E. HIS et R. ROBERT, 1980 et R. ROBERT et E. HIS, 1981). Les méthodes chimiques actuelles ne sont pas assez sensibles pour permettre le dosage de ce produit dans l'eau de mer, au seuil de toxicité pour les larves d'huitres. Une échelle d'action pour douze concentrations comprises entre 100 ug/l. et 0,02 ug/ l., sur les véligères de Crassostrea gigas est proposée. Elle permet d’évaluer le degré de contamination de l'eau de mer d'un part de plaisance et son évolution.
Tipo: Text
Ano: 1983 URL: http://archimer.ifremer.fr/doc/00090/20162/17815.pdf
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Etudes sur les causes de la perturbation de la reproduction et du développement larvaire de Crassostrea gigas dans le bassin d'Arcachon ArchiMer
Robert, Rene.
Centre autonome de production, d'élevage et de commercialisation, le bassin d'Arcachon assurait ces dernières années une part non négligeable de l'approvisionnement en naissain d'huîtres creuses des régions conchylicoles du littoral septentrional et méditerranéen de la France.
Tipo: Text
Ano: 1983 URL: http://archimer.ifremer.fr/doc/00090/20149/17799.pdf
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Etudes sur les mortalites larvaires de la coquille st Jacques Pecten maximus en ecloserie ArchiMer
Robert, Rene; Miner, Philippe; Nicolas, Jean-louis; Mazuret, Michel; Connan, Jean-paul.
Larval mortalities occurring in molluscan hatcheries have often been associated with bacterial contamination. Although batches of oyster and clam larvae have been routinely reared in the hatchery of Argenton without antibiotics, high larval mortalities have been recorded with Pecten maxirnus under similar conditions. For this species, an addition of chloramphenicol was found necessary. However, this chemical has now been banned in Europe. Thus either substitition products or an improvement in the rearing procedures is essential. Studies carried out have shown that neither a decrease in larval density (1 larvae. ml"l) nor sea water change frequency (1 per day) had any positive effects. Furthermore, elective substances such as sugars were not suitable and...
Tipo: Text Palavras-chave: Mortality; Pecten maximus; Hatchery; Larvae; Mollusc; Mortalité; Pecten maximus; Ecloserie; Larve; Mollusque.
Ano: 1995 URL: https://archimer.ifremer.fr/doc/1995/rapport-1568.pdf
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Evaluation of toxicity of Deepwater Horizon slick oil on spat of the oyster Crassostrea virginica ArchiMer
Vignier, Julien; Rolton, Anne; Soudant, Philippe; Chu, Fu-lin E.; Robert, Rene; Volety, Aswani K..
The 2010 explosion of the Deepwater Horizon (DWH) oil rig generated the largest marine oil spill in US history with millions of barrels of crude oil released in the Gulf of Mexico (GoM). The eastern oyster, Crassostrea virginica, is an ecologically and economically important species in the northern GoM. Due to its biological characteristics (sessile, filter feeding), juvenile oysters may have been affected. This study investigated the effects of surface-collected DWH oil prepared as high-energy water-accommodated fraction (HEWAF) on the survival of 2-month-old oyster spat, and evaluated the potential impacts of HEWAF on particle clearance rate and spat tissue. Exposure of oysters to a range of oil/HEWAF (0-7-66-147-908-3450 mu g tPAH50 (sum of 50...
Tipo: Text Palavras-chave: Oyster spat; Deepwater Horizon oil; Sublethal; Tissue; Inflammatory response; Histopathology.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00425/53615/54548.pdf
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Evolution spatio-temporelle des paramètres hydrobiologiques dans le bassin d'Arcachon (juillet 1984-juillet 1985) ArchiMer
Robert, Rene; Guillocheau, N..
L'évolution des paramètres hydrobiologiques du bassin d'Arcachon a été étudiée de juillet 1984 à juillet 1985. Ces observations confèrent à cette période un caractère exceptionnel pour la baie.
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Ano: 1987 URL: http://archimer.ifremer.fr/doc/00081/19232/16827.pdf
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Experimental shift of diet and DIC stable carbon isotopes: Influence on shell delta C-13 values in the Manila clam Ruditapes philippinarum ArchiMer
Poulain, C.; Lorrain, Anne; Mas, R.; Gillikin, D. P.; Dehairs, F.; Robert, Rene; Paulet, Yves-marie.
The influences of diet and seawater dissolved inorganic carbon (DIC) on the carbon isotope composition of shell aragonite (Cinched in the Manila clam Ruditapes philippinarum reared under laboratory conditions were investigated. Clams were exposed to two successive negative carbon isotope shifts: a first shift in diet (delta C-13(phytoplankton)) and a second shift, 35 days later, in DIC (delta C-13(DIC)). Both successive shifts induced a decrease in delta C-13(shell). These results are the first to experimentally confirm an incorporation of respired carbon derived from food and carbon from DIC into shell carbonate of adult bivalves. Skeletal delta C-13 responded to changes in the delta C-13 of both diet and DIC in less than 7 days. Consequently, proxies...
Tipo: Text Palavras-chave: Delta C-13(shell); DIC; Salinity; Metabolic carbon; Ruditapes philippinarum; Estuarine water; Proxy.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00003/11443/8001.pdf
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Growth, fatness and gross biochemical composition of the japanese oyster Crassostrea gigas in stanway cylinders in the bay of Arcachon, France ArchiMer
Robert, Rene; Trut, Gilles; Borel, Michel; Maurer, Daniele.
The Stanway cylinder was tested in the bay of Arcachon from September 1989 to August 1991. Trials were mainly performed on the Japanese oyster Crassostrea gigas in a wind-exposed area. The seed, spat, 18 month old oysters development and fattening of 2 year old oysters were studied. Compared to plastic bags the Stanway cylinder has high positive effects on survival and growth of Crassostrea gigas seed. It also has positive effects on commercial sized Crassostrea gigas fattening. Nevertheless, the cylinder has negative effects on the growth of Crassostrea gigas spat and 18 month old oysters, except for width. However, oyster quality is greatly improve : higher condition index, higher carbohydrate contents and higher shell densities were recorded. Because...
Tipo: Text Palavras-chave: Bay of Arcachon; Culture methods; Growth; Crassostrea gigas; Bassin d'Arcachon; Technique d'élevage; Croissance; Crassostrea gigas.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/rapport-1644.pdf
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Impact des facteurs anthropiques sur le recrutement de l'huître : l'exemple du bassin d'Arcachon ArchiMer
His, Edouard; Robert, Rene.
"Il est impossible d'imaginer un lieu plus favorable à l'ostréiculture que le bassin d'Arcachon" (Yonge, 1950). En effet, l'huître indigène, Ostrea edulis, et l'espèce plus récemment introduite, Crassostrea gigas, peuvent s'y reproduire en abondant. Si l'exploitation de la première a pris fin à la suite de deux épizooties consécutives, la seconde est l'objet d'une culture intensive dont la production annuelle est d'environ 12 000 tonnes de mollusques commercialisés. Des anomalies ont sévi dans la baie, de 1977 à 1981, rendant les récoltes de naissain pratiquement sensibles aux facteurs d'agression, et ont même été proposées par Woelke (1967) comme matériel biologique, afin de tester la qualité des eaux en zone littorale. La technique des élevages larvaires...
Tipo: Text Palavras-chave: Bassin d'Arcachon; Crassostrea gigas; Larves; Température; Nutrition; Bioessais; Facteurs anthropiques.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00019/13055/10013.pdf
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Impact of temperature on larval development and evolution of physiological indices in Crassostrea gigas ArchiMer
Ben Kheder, Rym; Moal, Jeanne; Robert, Rene.
The effect of four temperatures, ranging between 17 degrees C and 32 degrees C, was studied on development and lipid reserve management of Crassostrea gigas larvae. No effect of temperature was found on larval mortality, as high survival (>90%) was recorded before competence at all temperatures studied. Temperature did, nonetheless, have a strong effect on growth and settlement success. At low temperature (17 degrees C), larvae competent to metamorphose were only observed from day 23 and only a low percentage finally achieved metamorphosis (12%). The opposite was seen at temperatures >= 27 degrees C: larval competence appeared at day 18 and led to high rates of metamorphosis (60-90%). This difference at settlement seemed to be linked to larval...
Tipo: Text Palavras-chave: Crassostrea gigas; Larvae; Growth; Metamorphosis; Temperature; Lipids; Physiological indices.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00019/13046/10146.pdf
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Impacts of Deepwater Horizon oil and associated dispersant on early development of the Eastern oyster Crassostrea virginica ArchiMer
Vignier, J.; Donaghy, L.; Soudant, P.; Chu, F. L. E.; Morris, J. M.; Carney, M. W.; Lay, C.; Krasnec, M.; Robert, Rene; Volety, A. K..
The explosion of the Deepwater Horizon (DWH) oil platform resulted in large amounts of crude oil and dispersant Corexit 9500A® released into the Gulf of Mexico and coincided with the spawning season of the oyster, Crassostrea virginica. The effects of exposing gametes and embryos of C. virginica to dispersant alone (Corexit), mechanically (HEWAF) and chemically dispersed (CEWAF) DWH oil were evaluated. Fertilization success and the morphological development, growth, and survival of larvae were assessed. Gamete exposure reduced fertilization (HEWAF: EC201 h = 1650 μg tPAH50 L− 1; CEWAF: EC201 h = 19.4 μg tPAH50 L− 1; Corexit: EC201 h = 6.9 mg L− 1). CEWAF and Corexit showed a similar toxicity on early life stages at equivalent nominal concentrations....
Tipo: Text Palavras-chave: Deepwater Horizon oil; Oyster; Fertilization; CEWAF; Corexit; PAH.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00277/38853/42693.pdf
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Individual monitoring of gonad development in the European flat oyster Ostrea edulis by in vivo magnetic resonance imaging ArchiMer
Davenel, Armel; Gonzalez, Ricardo; Suquet, Marc; Quellec, Stephane; Robert, Rene.
Previous studies have shown that magnetic resonance imaging is a very appropriate non-invasive technique for quantifying the growth of somatic and gonadic tissues and sex determination in the Pacific oyster, Crassostrea gigas. Despite a thinner gonad, harder to distinguish than that of the Pacific oyster, we showed in this study that it was possible to distinguish and quantify the development of the gonad of the European flat oyster, Ostrea edulis, using a 3D MRI sequence with a suitable finer resolution. Compared to T2-weighted images, theoretically the most appropriate for good anatomical description, T1-weighted images were more suited to gonad observation. The gonad development was quantified by the number of voxels with higher grey level. Larvae were...
Tipo: Text Palavras-chave: Oyster; Ostrea edulis; Gonad development; Magnetic resonance imaging.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12508/9363.pdf
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Influence of diet assemblage on Ostrea edulis broodstock conditioning and subsequent larval development ArchiMer
Gonzalez, Ricardo; Mingant, Christian; Petton, Bruno; Robert, Rene.
In contrast with the Japanese oyster Crassostrea gigas and the Manila clam Ruditapes philippinarum, Ostrea edulis seed production in the hatchery has been reported to be erratic, with sudden and unexplained larval and post-metamorphosis mortalities. Fecundity and initial larval quality have been related to broodstock conditioning, but effects on larval development and metamorphosis remain poorly understood. In addition, molluscan larval mortalities have been often associated with bacterial contamination and flow-through techniques may help to overcome this problem. Both aspects have been considered in the present work. O. edulis broodstock were conditioned at 19 °C and fed three different microalgal diets. Two were single-species diets: Rhodomonas salina...
Tipo: Text Palavras-chave: Ostrea edulis; Feeding; Broodstock conditioning; Larval development; Flow-through.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00090/20093/17732.pdf
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Influence of feeding regime and temperature on development and settlement of oyster Ostrea edulis (Linnaeus, 1758) larvae ArchiMer
Robert, Rene; Vignier, Julien; Petton, Bruno.
Under controlled conditions of food density and temperature, larval performances (ingestion, growth, survival and settlement success) of the flat oyster, Ostrea edulis, were investigated using a flow-through rearing system. In the first experiment, oyster larvae were reared at five different phytoplankton densities (70, 500, 1500, 2500 and 3500 μm3 μL−1: ≈1, 8, 25, 42 and 58 cells μL−1 equivalent TCg), and in the second, larvae were grown at four different temperatures (15, 20, 25 and 30°C). Overall, larvae survived a wide range of food density and temperature, with high survival recorded at the end of the experiments. Microalgae concentration and temperature both impacted significantly larval development and settlement success. A mixed diet of Chaetoceros...
Tipo: Text Palavras-chave: Ostrea edulis; Larvae; Temperature; Feeding regime; Flow-through; DEB parameters.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00377/48817/49295.pdf
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Influence of food density and temperature on ingestion, growth and settlement of Pacific oyster larvae, Crassostrea gigas ArchiMer
Rico Villa, Benjamin; Pouvreau, Stephane; Robert, Rene.
Ingestion, growth and metamorphosis of Pacific oyster, Crassostrea gigas, larvae were studied under controlled conditions of food density and temperature using a combination of a flow-through rearing system and a hydrobiological monitoring device. In a first experiment larvae were exposed to three different phytoplankton densities (12, 20 and 40 cells μl-1) while in a second trial larvae were reared at five different temperatures (17, 22, 25, 27 and 32°C). Both food concentration and temperature significantly affected the larval physiology throughout the entire development from D-veliger to young spat. Larvae survived over a wide range of both environmental parameters with high survival at the end of experiments. The feeding functional response...
Tipo: Text Palavras-chave: Temperature; Food; Growth; Ecophysiology; Bivalve larvae; Crassostrea gigas.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2008/publication-4747.pdf
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Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition ArchiMer
Da Costa, F.; Petton, Bruno; Mingant, Christian; Bougaran, Gael; Rouxel, Catherine; Quere, Claudie; Wikfors, G. H.; Soudant, P.; Robert, Rene.
Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93–95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31–33%) and total fatty acids (4.0–4.7 pg cell−1). Larval oyster survival and growth rate...
Tipo: Text Palavras-chave: Larvae; Lipids; Oyster; Tisochrysis lutea.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00251/36258/36063.pdf
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Influence of phytoplankton diet mixtures on microalgae consumption, larval development and settlement of the Pacific oyster Crassostrea gigas (Thunberg) ArchiMer
Rico Villa, Benjamin; Le Coz, Jean-rene; Mingant, Christian; Robert, Rene.
Microalgae commonly used as feed for bivalves, Pavlova lutheri (P), Isochrisis affinis galbana (T) and Chaetoceros calcitrans forma pumilum (Cp), were fed to Pacific oyster Crassostrea gigas to assess their nutritional value for larval development and metamorphosis during two experiments. Monospecific, bispecific and trispecific diets were firstly evaluated during 3 weeks from D larvae to young postlarvae. Then bispecific diets, based on different T and Cp proportions, were assessed during a similar period. Concurrently, ingestion was studied through the whole larval and postlarval development for each diet and/or diet mixture. Because lipids are assumed to be a key nutrient for bivalves, biochemical analysis was undertaken on the second set of trials...
Tipo: Text Palavras-chave: Lipids; Grazing; Metamorphosis; Growth; Larvae; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1707.pdf
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Influence of sole and plurispecific diets on larval consumption and development of Pacific oyster Crassostrea gigas (Thunberg) ArchiMer
Robert, Rene; Rico Villa, Benjamin; Mingant, Christian.
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Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-6695.pdf
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Influence of temperature on disease transmission associated with ostreid herpes virus OSHV-1 µVAR in relation to survival of juvenile Crassostrea gigas ArchiMer
Petton, Bruno; Pernet, Fabrice; Robert, Rene; Boudry, Pierre; Alunno-bruscia, Marianne.
Since 2008, mass mortalities of 1-yr-old oysters Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have occurred along all coasts of France. These mortality events represent the most serious crisis for the French oyster industry since the introduction of C. gigas in the early 1970s. Temperature is commonly one of the major triggering factors of disease epizootics, notably for aquatic species. In France, OsHV-1 is generally detected in dying oysters when seawater temperature is higher than 16° C. The influence of temperature on OsHV-1 detection and expression was also demonstrated for C. gigas larvae and suspected for juveniles. The present study aims to characterize the effect of temperature on disease transmission and survival related...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00151/26259/24343.pdf
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In-situ microcosms, a tool for assessment of pesticide impacts on oyster spat (Crassostrea gigas) ArchiMer
Stachowski Haberkorn, S; Quiniou, Francoise; Nedelec, M; Robert, Rene; Limon, G; De La Broise, D.
Effects of the herbicide Basamais (bentazon) and the fungicide Opus (epoxiconazole) on oyster spat (Crassostrea gigas) were assessed using in-situ microcosms in a field experiment lasting 13 days. Six-week-old hatchery spat (mean size 1.1 mm), previously collected on PVC plates, was immersed in glass bottles filled with 200 mu m filtered seawater. Bottles were maintained underwater at 6 m depth and their water content changed every other day. Growth, measured as shell area index increase, was 126 +/- 4% in the control bottles. While no growth differences were observed between control and individual pesticide treatments at 10 mu g l(-1), oysters treated with a mix of 10 mu g l(-1) Opus and 10 mu g l(-1) Basamais showed a 50% growth reduction compared with...
Tipo: Text Palavras-chave: Epoxiconazole; Bentazon; Pesticide; Toxicity; Oyster; Microcosm.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4134.pdf
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